RESUMO
ABSTRACT: Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed to investigate a possible relationship between fetuin-A gene polymorphism and the presence of dental calculus. A possible relationship between serum, saliva and gingival crevicular fluid (GCF) levels of fetuin-A was also investigated. Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with or without dental calculus. Additionally, serum, saliva and GCF fetuin-A levels of patients were compared according to dental calculus presence. A significant difference was not observed in the distribution of the fetuin-A c.742C > T and c.766C > G polymorphisms between patients with or without dental calculus. Saliva and GCF fetuin-A concentrations of patients with dental calculus were statistically higher than those without dental calculus (P=0.001, P=0.036 respectively). According to our results, fetuin-A c.742C > T and c.766C > G polymorphisms were not associated with presence of dental calculus. However, higher GCF and saliva fetuin-A levels were detected in patients with dental calculus than in patients without dental calculus, which may result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation.
Assuntos
Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Polimorfismo Genético , Saliva/química , Cálculos Dentários/química , Líquido do Sulco Gengival/química , alfa-2-Glicoproteína-HS/análise , alfa-2-Glicoproteína-HS/genética , Valores de Referência , Saliva/fisiologia , Ensaio de Imunoadsorção Enzimática , Cálculos Dentários/fisiopatologia , Cálculos Dentários/genética , Estudos de Casos e Controles , Análise de Variância , Líquido do Sulco Gengival/fisiologia , Estatísticas não Paramétricas , Placa Dentária/química , Estudos de Associação Genética , Genótipo , Pessoa de Meia-IdadeRESUMO
Aim: The aim of the present study was to determine age- and gender-related values for healthy mice of CD3+ T and CD19+ B lymphocytes and CD3+/ CD19+ (T/B) ratios in peripheral blood and gingival tissue by the flow cytometry technique. Methods: The study was carried out on periodontally healthy 60 BALB/c mice. They were divided into five groups according to the their age (newborn, weaning, puberty, adult and elder). Males and females were equally represented in each group. CD3+ and CD19+ T lymphocytes and CD3+/CD19+ ratio values in gingival tissue and peripheral blood were determined using flow cytometry in the biopsy samples. Results: There were no significant differences in the CD3+ and CD19+ lymphocytes, and CD3+/ CD19+ ratios in gingival tissue for all age groups (p>0.05). Mean relative number of peripheral blood CD3+ T lymphocyte indicated a decrease in puberty group compared to the other groups (p<0.05), while relative number of peripheral blood CD19+ B lymphocyte increased in adult and aged group. The peripheral blood CD3+/ CD19+ T/B lymphocytes ratios decreased in adult and aged group. Conclusions: The results of the present study showed that differences were present in periods of life and gender in peripheral blood of mice. Moreover,significantly differences were found between genders in gingival tissue.